Anti-EGF Antibody (9E161)

Name: Anti-EGF Antibody (9E161)
Brand: TargetMol
SKU: TMAY-03835
Rating: 4.5 (1 reviews)

😃Good

Catalog No. TMAY-03835

Alias epidermal growth factor

Anti-EGF Antibody (9E161) is a Mouse antibody targeting EGF. Anti-EGF Antibody (9E161) can be used in ELISA.

Anti-EGF Antibody (9E161)

😃Good

Catalog No. TMAY-03835Alias epidermal growth factor

Anti-EGF Antibody (9E161) is a Mouse antibody targeting EGF. Anti-EGF Antibody (9E161) can be used in ELISA.

Pack Size	Price	Availability	Quantity
100 μL	$198	7-10 days

Bulk & Custom

Add to Cart

Questions

Resource Download

Instruction Manual of Detection Antibody

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity

Description	Antibody Type: Mouse Monoclonal Application: ELISA Reactivity: Rat
Alias	epidermal growth factor
Ig Type	Mouse IgG1
Clone	9E161
Reactivity	Rat
Application	ELISA
Recommended Dose	ELISA: 1:1000-1:2000
Antibody Type	Monoclonal
Host Species	Mouse
Construction	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat Epidermal Growth Factor / EGF (rR Epidermal Growth Factor / EGF; TMPY-04220; NP_036974.1; Asn974-Arg1026). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification	Protein A
Appearance	Liquid
Formulation	0.2 μm filtered solution in PBS
Research Background	EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.

Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	Recombinant Protein: Rat Epidermal Growth Factor / EGF Protein (TMPY-04220)
Antigen Species	Rat

Chemical Properties

Stability & Storage

Stability & Storage	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
Transport	Shipping with blue ice.

Sci Citations

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc