ROBO1 Protein, Human, Recombinant (hFc)

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Catalog No. TMPY-06346

ROBO1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 98.89 kDa and the accession number is Q9Y6N7-1.

ROBO1 Protein, Human, Recombinant (hFc)

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Catalog No. TMPY-06346

ROBO1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 98.89 kDa and the accession number is Q9Y6N7-1.

Pack Size	Price	USA Warehouse	Global Warehouse
5 μg	$68	7-10 days	7-10 days
10 μg	$108	7-10 days	7-10 days
20 μg	$178	7-10 days	7-10 days
50 μg	$359	7-10 days	7-10 days
100 μg	$696	7-10 days	7-10 days
200 μg	$1,060	7-10 days	7-10 days
500 μg	$2,230	7-10 days	7-10 days
1 mg	$4,530	7-10 days	7-10 days

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In Stock Estimated shipping dateUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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User Guide of Recombinant Proteins

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Biological Activity	1.Immobilized Recombinant Human SLIT2 Protein (His Tag) (Cat#TMPY-07086) at 10 μg/mL (100 μL/well) can bind Recombinant Human ROBO1 Protein (Fc Tag) (Cat#TMPY-06346), the EC50 is 60-180 ng/mL. 2.Loaded Recombinant Human ROBO1 Protein, hFc Tag (Cat#TMPY-06346) on proA Biosensor, can bind Recombinant Human SLIT2 Protein, His Tag (Cat#TMPY-07086) with an affinity constant of 42.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Description	ROBO1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 98.89 kDa and the accession number is Q9Y6N7-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q9Y6N7-1
Synonyms	SAX3,DUTT1
Construction	A DNA sequence encoding the human ROBO1 (Q9Y6N7-1) (Met1-Pro897) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: GIn 26
Protein Purity	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Molecular Weight	98.89 kDa (predicted); 136.91 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.