Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, IF, FCM

Reactivity: Human

Mouse IgG2a

8S156

Human

1. Immunofluorescence staining of hela cell with TMAH-00991 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
2. Immunofluorescence staining of SH-SY5Y cell with TMAH-00991 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
3. Overlay Peak curve showing Hela cells surface stained with TMAH-00991 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,026 events was performed.

ELISA, IF, FCM

IF:1:20-1:200; FCM:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human PLAUR Protein

Human

5329

Cancer, Cardiovascular, Immunology