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Anti-uPAR/PLAUR Antibody (8S156) is an antibody targeting uPAR/PLAUR. Anti-uPAR/PLAUR Antibody (8S156) can be used in ELISA, IF, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Mouse Monoclonal Application: ELISA, IF, FCM Reactivity: Human |
Alias | U-PAR, uPAR, PLAUR, MO3, CD87 |
Ig Type | Mouse IgG2a |
Clone | 8S156 |
Reactivity | Human |
Verified Activity | 1. Immunofluorescence staining of hela cell with TMAH-00991 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). 2. Immunofluorescence staining of SH-SY5Y cell with TMAH-00991 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). 3. Overlay Peak curve showing Hela cells surface stained with TMAH-00991 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,026 events was performed. |
Application | ELISA, IF, FCM |
Recommended Dose | IF:1:20-1:200; FCM:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane. Cell projection, invadopodium membrane.; [Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 2]: Secreted. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human PLAUR Protein |
Antigen Species | Human |
Gene ID | 5329 |
Uniprot ID | |
Biology Area | Cancer, Cardiovascular, Immunology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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