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Anti-uPAR/PLAUR Antibody (8S156)

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Catalog No. TMAH-00991
Alias U-PAR, uPAR, PLAUR, MO3, CD87

Anti-uPAR/PLAUR Antibody (8S156) is an antibody targeting uPAR/PLAUR. Anti-uPAR/PLAUR Antibody (8S156) can be used in ELISA, IF, FCM.

Anti-uPAR/PLAUR Antibody (8S156)

Anti-uPAR/PLAUR Antibody (8S156)

😃Good
Catalog No. TMAH-00991Alias U-PAR, uPAR, PLAUR, MO3, CD87
Anti-uPAR/PLAUR Antibody (8S156) is an antibody targeting uPAR/PLAUR. Anti-uPAR/PLAUR Antibody (8S156) can be used in ELISA, IF, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, IF, FCM

Reactivity: Human
AliasU-PAR, uPAR, PLAUR, MO3, CD87
Ig Type
Mouse IgG2a
Clone
8S156
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of hela cell with TMAH-00991 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
2. Immunofluorescence staining of SH-SY5Y cell with TMAH-00991 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
3. Overlay Peak curve showing Hela cells surface stained with TMAH-00991 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,026 events was performed.
Application
ELISA, IF, FCM
Recommended Dose
IF:1:20-1:200; FCM:1:20-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane. Cell projection, invadopodium membrane.; [Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 2]: Secreted.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Research BackgroundActs as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human PLAUR Protein
Antigen Species
Human
Gene ID
5329
Uniprot ID
Biology Area
Cancer, Cardiovascular, Immunology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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