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Anti-Renin Antibody (8M814)

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Catalog No. TMAY-01384
Alias renin, REN, HNFJ2, FLJ10761

Anti-Renin Antibody (8M814) is a Rabbit antibody targeting Renin. Anti-Renin Antibody (8M814) can be used in ELISA,IHC-P,FCM,ICC/IF.

Anti-Renin Antibody (8M814)

Anti-Renin Antibody (8M814)

😃Good
Catalog No. TMAY-01384Alias renin, REN, HNFJ2, FLJ10761
Anti-Renin Antibody (8M814) is a Rabbit antibody targeting Renin. Anti-Renin Antibody (8M814) can be used in ELISA,IHC-P,FCM,ICC/IF.
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100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Renin Antibody (8M814) is a Rabbit antibody targeting Renin. Anti-Renin Antibody (8M814) can be used in ELISA,IHC-P,FCM,ICC/IF.
Aliasrenin, REN, HNFJ2, FLJ10761
Ig Type
Monoclonal Rabbit IgG
Clone
8M814
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Renin monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Immunochemical staining of human RENIN in human kidney with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

3. Flow cytometric analysis of Human Renin expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
ELISA,IHC-P,FCM,ICC/IF
Recommended Dose
ELISA: 1:25000-1:50000; IHC-P: 1:500-1:2000; ICC-IF: 1:100-1:500; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Renin (rh Renin; TMPY-00908; NP_000528.1; Met 1-Arg 406).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundRenin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human Renin protein (TMPY-00908)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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