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Anti-Renin Antibody (8M814)
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Catalog No. TMAY-01384
Alias renin, REN, HNFJ2, FLJ10761
Anti-Renin Antibody (8M814) is a Rabbit antibody targeting Renin. Anti-Renin Antibody (8M814) can be used in ELISA,IHC-P,FCM,ICC/IF.
Anti-Renin Antibody (8M814)
Anti-Renin Antibody (8M814)
😃Good
Catalog No. TMAY-01384Alias renin, REN, HNFJ2, FLJ10761
Anti-Renin Antibody (8M814) is a Rabbit antibody targeting Renin. Anti-Renin Antibody (8M814) can be used in ELISA,IHC-P,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, IHC-P, FCM, ICC/IF Reactivity: Human |
| Alias | renin, REN, HNFJ2, FLJ10761 |
| Ig Type | Rabbit IgG |
| Clone | 8M814 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Renin monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Immunochemical staining of human RENIN in human kidney with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. 3. Flow cytometric analysis of Human Renin expression on Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human Renin, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,IHC-P,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:25000-1:50000; IHC-P: 1:500-1:2000; ICC-IF: 1:100-1:500; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Renin (rh Renin; TMPY-00908; NP_000528.1; Met 1-Arg 406). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Renin protein (TMPY-00908) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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