EphA4 Protein, Human, Recombinant (aa 570-986, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75 kDa and the accession number is P54764.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 μg | 5 days | $ 498.00 |
Description | EphA4 Protein, Human, Recombinant (aa 570-986, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75 kDa and the accession number is P54764. |
Species | Human |
Expression System | Baculovirus Insect Cells |
Tag | His, GST |
Accession Number | P54764 |
Synonyms | EPH receptor A4, TYRO1, HEK8, SEK |
Construction | The Human EPHA4 (P54764) (Ser570-Val986) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Protein Purity | > 90 % as determined by SDS-PAGE |
Molecular Weight | 75 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.5, 10% glycerol, 3 mM DTT. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
Research Background | EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. |
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Please read the User Guide of Recombinant Proteins for more specific information.
EphA4 Protein, Human, Recombinant (aa 570-986, His & GST) EPH receptor A4 HEK 8 TYRO1 HEK8 TYRO-1 SEK TYRO 1 HEK-8 recombinant recombinant-proteins proteins protein