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Anti-B2M/beta 2-Microglobulin Antibody (7P459)
🥰Excellent
Catalog No. TMAY-02179
Anti-B2M/beta 2-Microglobulin Antibody (7P459) is a Mouse antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (7P459) can be used in WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF.
Anti-B2M/beta 2-Microglobulin Antibody (7P459)
Anti-B2M/beta 2-Microglobulin Antibody (7P459)
🥰Excellent
Catalog No. TMAY-02179
Anti-B2M/beta 2-Microglobulin Antibody (7P459) is a Mouse antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (7P459) can be used in WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF.
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Chemical Properties
| Description | Anti-B2M/beta 2-Microglobulin Antibody (7P459) is a Mouse antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (7P459) can be used in WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 7P459 |
| Reactivity | Human |
| Verified Activity | 1. Anti-B2M mouse monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell Lysate. -Lane B: U937 Whole Cell Lysate. -Lane C: Raji Whole Cell Lysate. -Lane D: HeLa Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:14 kDa. -Observed band size:14 kDa. 2. Immunochemical staining of human B2M in human ovarian cancer with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human B2M in human skin with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections. 4. Immunofluorescence staining of B2M in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human B2M monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane. 5. Flow cytometric analysis of Human B2M / Beta-2-microglobulin expression on human whole blood lymphocytes. Cells were stained with purified anti-Human B2M / Beta-2-microglobulin, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. 6. Anti-Beta-2 microglobulin mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Beta-2 microglobulin konckout Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:14 kDa. -Observed band size:14 kDa(Validation Experiment) |
| Application | WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IHC-P: 1:30-1:100; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Beta-2 microglobulin/B2M (rh Beta-2 microglobulin/B2M; TMPY-01686; NP_004039.1; Met1-Met119). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV. |
| Conjucates | Unconjugated |
| Others Formats | FITC/HRP |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Human Beta-2 microglobulin/B2M Protein (TMPY-01686) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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