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ROR1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 70.53 kDa and the accession number is NP_005003.2.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $600 | 7-10 days | |
100 μg | $1,390 | 7-10 days |
Biological Activity | Immobilized ROR1 Antibody, Rabbit PAb, Antigen Affinity Purified at 2 μg/mL (100 μL/well) can bind Recombinant Human ROR1 Protein (Fc & AVI Tag), Biotinylated , the EC50 is 1.1-3.3 ng/mL. |
Description | ROR1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 70.53 kDa and the accession number is NP_005003.2. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-hFc-Avi |
Accession Number | NP_005003.2 |
Synonyms | receptor tyrosine kinase-like orphan receptor 1,NTRKR1,dJ537F10.1 |
Construction | A DNA sequence encoding the Human ROR1 (NP_005003.2)(Met1-Glu403) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gln 30 |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Molecular Weight | 70.53 kDa (predicted); 85.43 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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