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Anti-GPC3 Antibody (5N458) is an antibody targeting GPC3. Anti-GPC3 Antibody (5N458) can be used in ELISA, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Mouse Monoclonal Application: ELISA, FCM Reactivity: Human |
Alias | SGBS1, SGBS, SGB, SDYS, OCI-5, MXR7, GTR2-2, Glypican 3, DGSX |
Ig Type | Mouse IgG2a |
Clone | 5N458 |
Reactivity | Human |
Verified Activity | Overlay Peak curve showing THP-1 cells surface stained with TMAH-00504 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 010 events was performed. |
Application | ELISA, FCM |
Recommended Dose | FCM:1:20-1:500. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human GPC3 Protein |
Antigen Species | Human |
Gene ID | 2719 |
Uniprot ID | |
Biology Area | Cancer, Developmental biology, Signal transduction, Stem cells |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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