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Anti-GPC3 Antibody (5N458)
😃Good
Catalog No. TMAH-00504
Alias SGBS1, SGBS, SGB, SDYS, OCI-5, MXR7, GTR2-2, Glypican 3, DGSX
Anti-GPC3 Antibody (5N458) is an antibody targeting GPC3. Anti-GPC3 Antibody (5N458) can be used in ELISA, FC.
Anti-GPC3 Antibody (5N458)
Anti-GPC3 Antibody (5N458)
😃Good
Catalog No. TMAH-00504Alias SGBS1, SGBS, SGB, SDYS, OCI-5, MXR7, GTR2-2, Glypican 3, DGSX
Anti-GPC3 Antibody (5N458) is an antibody targeting GPC3. Anti-GPC3 Antibody (5N458) can be used in ELISA, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-GPC3 Antibody (5N458) is an antibody targeting GPC3. Anti-GPC3 Antibody (5N458) can be used in ELISA, FC. |
| Alias | SGBS1, SGBS, SGB, SDYS, OCI-5, MXR7, GTR2-2, Glypican 3, DGSX |
| Ig Type | Mouse IgG2a |
| Clone | 5N458 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing THP-1 cells surface stained with TMAH-00504 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 010 events was performed. |
| Application | ELISA, FC |
| Recommended Dose | FC:1:20-1:500. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signali... |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human GPC3 Protein |
| Antigen Species | Human |
| Gene ID | 2719 |
| Uniprot ID | |
| Biology Area | Cancer, Developmental biology, Signal transduction, Stem cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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