EGFR Protein, Mouse, Recombinant (His)

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD
100 μg	In stock	$ 386.00
200 μg	5 days	$ 682.00
500 μg	5 days	$ 1,440.00

Pack Size

Availability

Price/USD

Quantity

100 μg

In stock

$ 386.00

200 μg

5 days

$ 682.00

500 μg

5 days

$ 1,440.00

Biological Information

1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc , The EC50 of human EGF-Fc is 60-90 ng/mL. 3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc , The EC50 of mouse EGF-Fc is 70-100 ng/mL.

Description

Species	Mouse
Expression System	HEK293
Tag	His
Accession Number	Q01279
Synonyms	Errp, AI552599, Errb1, 9030024J15Rik, wa2, Erbb, wa-2, Wa5, epidermal growth factor receptor
Construction	A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
Molecular Weight	Approxiamtely 70.8 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy