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Anti-DNMT1 Antibody (2S499)
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Catalog No. TMAH-00360
Alias MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM
Anti-DNMT1 Antibody (2S499) is an antibody targeting DNMT1. Anti-DNMT1 Antibody (2S499) can be used in ELISA, IHC, IF.
Anti-DNMT1 Antibody (2S499)
Anti-DNMT1 Antibody (2S499)
😃Good
Catalog No. TMAH-00360Alias MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM
Anti-DNMT1 Antibody (2S499) is an antibody targeting DNMT1. Anti-DNMT1 Antibody (2S499) can be used in ELISA, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-DNMT1 Antibody (2S499) is an antibody targeting DNMT1. Anti-DNMT1 Antibody (2S499) can be used in ELISA, IHC, IF. |
| Alias | MCMT, M.HsaI, EC 2.1.1.37, DNMT, DNA MTase HsaI, DNA methyltransferase HsaI, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9, AIM |
| Ig Type | Rabbit IgG |
| Clone | 2S499 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00360 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of HepG2 Cells with TMAH-00360 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, IHC, IF |
| Recommended Dose | IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Dnmt1 |
| Antigen Species | Human |
| Gene ID | 1786 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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