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Anti-Iba1 Antibody (3Y490)
😃Good
Catalog No. TMAY-02698
Alias IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Anti-Iba1 Antibody (3Y490) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (3Y490) can be used in ELISA,ICC/IF,IHC-P.
Anti-Iba1 Antibody (3Y490)
Anti-Iba1 Antibody (3Y490)
😃Good
Catalog No. TMAY-02698Alias IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Anti-Iba1 Antibody (3Y490) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (3Y490) can be used in ELISA,ICC/IF,IHC-P.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 20 μL | $93 | 7-10 days | 7-10 days | |
| 100 μL | $269 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Iba1 Antibody (3Y490) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (3Y490) can be used in ELISA,ICC/IF,IHC-P. |
| Synonyms | IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1 |
| Ig Type | Rabbit IgG |
| Clone | 3Y490 |
| Reactivity | Human |
| Specificity | Human Iba1 |
| Verified Activity | 1. Immunochemical staining of human AIF1 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human AIF1 in human brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 3. Immunofluorescence staining of AIF1 in K562 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human AIF1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane. |
| Application | |
| Recommended Dose | ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; TMPY-03337; P55008-1; Met1-Pro147). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human IBA1 / AIF1 Protein (TMPY-03337) |
| Antigen Species | Human |
| Biology Area | Calcium-binding Proteins and Related Molecules |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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