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Siglec-10 Protein, Human, Recombinant (His & Avi), Biotinylated
(Synonyms: SLG2, SIGLEC10, PRO940, MGC126774) Copy Product InfoSynonyms: SLG2, SIGLEC10, PRO940, MGC126774
Catalog No. TMPK-00364 Copy Product Info
Siglec-10 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 61.13 kDa and the accession number is Q96LC7-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $91 | - | In Stock | |
| 10 µg | $148 | - | In Stock | |
| 20 µg | $245 | - | In Stock | |
| 50 µg | $483 | 7-10 days | 7-10 days | |
| 100 µg | $813 | - | In Stock | |
| 200 µg | $1,470 | 7-10 days | 7-10 days | |
| 500 µg | $3,250 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Biotinylated Human Siglec-10, His Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 81.3ng/ml determined by ELISA. |
| Description | Siglec-10 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 61.13 kDa and the accession number is Q96LC7-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q96LC7-1 |
| Construction | Met17-Thr546 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM Nacl, 200 mM Arginine (pH 8.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | SLG2, SIGLEC10, PRO940, MGC126774 |
| Research Background | Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system. |
| Molecular Weight | 61.13 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: Siglec-10 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | Siglec-10 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight
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