1. Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Biotinylated Human IL-2-His-Avi with an affinity constant of 8.60 nM as determined in BLI assay. (Regularly tested) 2. Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.2 ng/ml. (Regularly tested)3. Immobilized Human IL-2RA-His at 2μg/ml (100 μl/well)can bind Human IL-2-His-Avi. The ED50 of Human IL-2-His-Avi is 6.45 ng/ml. (Regularly tested)

IL-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 16-18 KDa and the accession number is P60568.

Human

HEK293 Cells

TCGF,T-Cell Growth Factor,Interleukin-2,IL-2,IL2,Aldesleukin

Ala21-Thr153

Ala21-Thr153

Greater than 90% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-2(IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system，belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.