Immobilized Anti-Siglec15 at 1.0 μg/ml (100 μl/well) can bind Siglec-15, hFc, Human with a EC 50 = 5.184 ng/ml and a linear range of 0.064−200.0 ng/ml when detected by Mouse Anti–Human IgG Fc-HRP.

Siglec-15 Protein, Human, Recombinant (hFc) is expressed in HEK293 Cells. The accession number is Q6ZMC9.

Human

HEK293 Cells

SIGLEC-15,HsT1361,CD33L3

Phe20-Thr263

> 90% as determined by SDS-PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Siglec-15 preferentially recognizes the Neu5Acalpha2-6GalNAcalpha- structure. Siglec-15 associates with the activating adaptor proteins DNAX activation protein (DAP)12 and DAP10 via its lysine residue in the transmembrane domain. Siglec-15 is the second human Siglec identified to have an activating signaling potential; unlike Siglec-14, however, it does not have an inhibitory counterpart.