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BTN3A1 Protein, Human, Recombinant (His)

Catalog No. TMPY-04844
Synonyms: BTN3.1, butyrophilin subfamily 3 member A1, BTF5, BT3.1, CD277

BTN3A1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.6 kDa and the accession number is O00481-2.

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BTN3A1 Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
100 μg In stock $ 451.00
200 μg 5 days $ 761.00
500 μg 5 days $ 1,510.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Testing in progress
Description BTN3A1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.6 kDa and the accession number is O00481-2.
Species Human
Expression System HEK293 Cells
Tag C-His
Accession Number O00481-2
Synonyms BTN3.1, butyrophilin subfamily 3 member A1, BTF5, BT3.1, CD277
Construction The Human BTN3A1 (NP_919423.1) (Met1-Gly254) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity > 95 % as determined by SDS-PAGE.

Molecular Weight 25.6 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background BTN3A1 has the structure of a type I receptor of the Ig superfamily and is part of a family of seven BTN receptors encoded by genes in the MHC. BTN molecules are composed of two Ig domains (IgV, IgC2), a single transmembrane domain, and a large carboxyl-terminal domain termed B3.2 (or PRYSPRY) located in the cell cytoplasm. There are three human BTN3A loci, BTN3A1, BTN3A2, and BTN3A3, and clear orthologs of BTN3A molecules, now called CD277, are absent from the mouse genome. Despite its similarity to B7 molecules, BTN3A1 was proposed to act not as a coreceptor or costimulatory molecule, but rather to directly present pAg to the γδ TCR in a manner analogous to MHC-restricted peptide presentation. However, this model of BTN3A1 function has been challenged by conflicting data, which show pAg binding to a positively charged pocket in the cytosolic B3.2 domain, and that BTN3A1 does not directly engage the γδ TCR. This contradictory picture has emerged as a result of the complexity of the system and in particular by the use of endogenous and exogenous routes of Ag delivery in in vitro assays.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

References and Literature

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Keywords

BTN3A1 Protein, Human, Recombinant (His) BTN3.1 butyrophilin subfamily 3 member A1 BTF 5 BTF-5 CD-277 CD 277 BTF5 BT3.1 CD277 recombinant recombinant-proteins proteins protein

 

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