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Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) is an antibody targeting Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602). Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) can be used in ELISA, WB, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
Alias | p-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602), p-PAK4/PAK5/PAK6 (S474/S560/S602), Phospho-PAK4/PAK5/PAK6 (S474/S560/S602), PAK4/PAK5/PAK6 (p-Ser474/Ser560/Ser602), PAK4/PAK5/PAK6 (p-S474/S560/S602) |
Ig Type | Rabbit IgG |
Clone | 4J12 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not) -All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 64,75,80 KDa -Observed band size: 75,80 KDa 2. Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with TMAH-00938 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, WB, IF |
Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) |
Antigen Species | Human |
Uniprot ID | |
Biology Area | Cell Biology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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