SOST Protein, Human, Recombinant (His), Biotinylated

SOST Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is Q9BQB4-1.

Pack Size	Availability	Price/USD	Quantity
20 μg	5 days	$ 383.00
100 μg	5 days	$ 913.00

Pack Size

Availability

Price/USD

Quantity

20 μg

5 days

$ 383.00

100 μg

5 days

$ 913.00

Description

SOST Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is Q9BQB4-1.

Species	Human
Expression System	HEK293 Cells
Tag	N-His
Accession Number	Q9BQB4-1
Synonyms	VBCH, SOST1, DAND6, sclerostin, CDD
Construction	The SOST (NP_079513.1) (Gln24-Tyr213) was expressed with a polyhistidine tag at the N-terminus. The purified protein was biotinylated in vitro.
Protein Purity	> 90 % as determined by SDS-PAGE.
Molecular Weight	22.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing Sterile PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Sclerostin, the protein product of the SOST gene, is a potent inhibitor of bone formation. Sclerostin protein is widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days, and was originally identified as an important regulator of bone remodeling, homeostasis, and links bone resorption and bone apposition. Recent studies have revealed that Sclerostin protein inhibits the bone growth probably by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation.