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Anti-BRAF Antibody (1N184) (Synonyms: v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein ...)

Catalog No. TMAH-00118 Copy Product Info
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Anti-BRAF Antibody (1N184) is an antibody targeting BRAF. Anti-BRAF Antibody (1N184) can be used in ELISA,IHC.

Anti-BRAF Antibody (1N184)

Copy Product Info
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Catalog No. TMAH-00118
Synonyms v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p94, EC 2.7.11.1, BRAF1

Anti-BRAF Antibody (1N184) is an antibody targeting BRAF. Anti-BRAF Antibody (1N184) can be used in ELISA,IHC.

Anti-BRAF Antibody
(1N184)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2087-10 days7-10 days
100 μL$3477-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-BRAF Antibody (1N184) is an antibody targeting BRAF. Anti-BRAF Antibody (1N184) can be used in ELISA,IHC.
Synonymsv-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, p94, EC 2.7.11.1, BRAF1
Ig Type
Rabbit IgG
Clone
1N184
Reactivity
Human
Verified Activity
1. IHC image of TMAH-00118 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
2. IHC image of TMAH-00118 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
Recommended Dose
IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus. Cytoplasm. Cell membrane.
Tissue SpecificityBrain and testis.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundProtein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human B Raf
Antigen Species
Human
Gene Name
BRAF
Gene ID
Uniprot ID
Biology Area
Cancer, Cell biology, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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