Assay #1: Immobilized PD-L1, hFc, Human (Cat. No.: Z03371) at 1.0 μg/ml (100 μl/well) can bind biotinylated PD-1, hFc, Human. Assay #2: Immobilized PD-L1, His, Human(Cat. No.: Z03425) at 2.0 μg/ml (100 µl/well) can bind PD-1, hFc, Human.

PD-1 Protein, Human, Recombinant (CHO, hFc) is expressed in CHO Cells. The accession number is Q15116.

Human

CHO Cells

Programmed cell death 1,PDCD1,PD-1,CD279

Leu25-Gln167

> 98% as determined by SDS-PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279) or PDCD1, is a protein that in humans is encoded by the PDCD1 gene. PD-1 is a cell surface receptor that belongs to the immunoglobulin superfamily and is expressed on T cells and pro-B cells.PD-1 binds two ligands, PD-L1 and PD-L2. PD-1 and its ligands play an important role in down regulating the immune system by preventing the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance. The inhibitory effect of PD-1 is accomplished through a dual mechanism of promoting apoptosis (programmed cell death) in antigen specific T-cells in lymph nodes while simultaneously reducing apoptosis in regulatory T cells (suppressor T cells)