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Anti-MBP tag Polyclonal Antibody
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Catalog No. TMAB-08651
Anti-MBP tag Polyclonal Antibody is a Rabbit antibody targeting MBP tag. Anti-MBP tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
Anti-MBP tag Polyclonal Antibody
Anti-MBP tag Polyclonal Antibody
😃Good
Catalog No. TMAB-08651
Anti-MBP tag Polyclonal Antibody is a Rabbit antibody targeting MBP tag. Anti-MBP tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $184 | 7-10 days | 7-10 days | |
| 500 μL | $757 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MBP tag Polyclonal Antibody is a Rabbit antibody targeting MBP tag. Anti-MBP tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| Reactivity | Species independent |
| Application | |
| Recommended Dose | ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Periplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein. |
| Immunogen | KLH conjugated synthetic peptide: E. coli Maltose Binding Protein |
| Gene Name | malE |
| Gene ID | |
| Protein Name | Maltose/maltodextrin-binding periplasmic protein |
| Function | Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody. |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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