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CD47 Protein, Human, Recombinant (aa 1-139, His)

Catalog No. TMPY-04935
Synonyms: MER6, CD47 molecule, IAP, OA3

CD47 Protein, Human, Recombinant (aa 1-139, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is Q08722-3.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CD47 Protein, Human, Recombinant (aa 1-139, His)
Pack Size Availability Price/USD Quantity
100 μg In stock $ 386.00
200 μg 5 days $ 682.00
500 μg 5 days $ 1,440.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information 1. Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha hFc at 2 μg/mL (100 μL/well) can bind Human CD47 His, the EC50 of Human CD47 His is 5.0-25.0 ng/mL.
2. Captured Ligufalimab (IgG4) on proA Chip can bind CD47 with an affinity constant of 10.82 nM as determined in a SPR assay (Routinely tested).
3. Loaded Hu5F9-G4 (IgG4) on ProA Biosensor, can bind human CD47 protein, His Tag with an affinity constant of 3.02 nM as determined in BLI assay (Routinely tested).

Description CD47 Protein, Human, Recombinant (aa 1-139, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is Q08722-3.
Species Human
Expression System HEK293 Cells
Tag C-His
Accession Number Q08722-3
Synonyms MER6, CD47 molecule, IAP, OA3
Construction The Human CD47 (NP_942088.1) (Met1-Pro139) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Molecular Weight 15.2 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

References and Literature

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Keywords

CD47 Protein, Human, Recombinant (aa 1-139, His) MER-6 OA-3 MER6 OA 3 CD47 molecule IAP MER 6 OA3 recombinant recombinant-proteins proteins protein

 

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