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FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated

(Synonyms: TK25, TK14, K-SAM, KGFR, JWS, fibroblast growth factor receptor 2, FGFR2 α(IIIb), ECT1, CFD1, CEK3, CD332, BFR-1, BEK, BBDS) Copy Product Info

Synonyms: TK25, TK14, K-SAM, KGFR, JWS, fibroblast growth factor receptor 2, FGFR2 α(IIIb), ECT1, CFD1, CEK3, CD332, BFR-1, BEK, BBDS

Catalog No. TMPY-06321 Copy Product Info
FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 42.88 kDa and the accession number is NP_075259.4.

Product Introduction

Bioactivity
Bioactivity
Immobilized Recombinant Human FGF10 / KGF2 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human FGFR2 Protein (alpha IIIb, ECD, His & AVI Tag), Biotinylated , the EC50 is 60-180 ng/mL.
Description
FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 42.88 kDa and the accession number is NP_075259.4.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberNP_075259.4
ConstructionA DNA sequence encoding the Human FGFR2 (NP_075259.4)(Met1-Glu378) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Arg 22
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTK25, TK14, K-SAM, KGFR, JWS, fibroblast growth factor receptor 2, FGFR2 α(IIIb), ECT1, CFD1, CEK3, CD332, BFR-1, BEK, BBDS
Research Background
Chemical Properties
Molecular Weight42.88 kDa (predicted); 70 kDa (reducing conditions)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Keywords

Related Tags: FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated chemical structure | FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated molecular weight