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P-Selectin Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P16109.
Pack Size | Price | Availability | Quantity |
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Biological Activity | Immobilized Human P-Selectin, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.9 ng/ml determined by ELISA. |
Description | P-Selectin Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P16109. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | P16109 |
Construction | Trp42-Ala771 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 81.05 kDa (Predicted); 110-140 kDa (Due to glycosylation) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. |
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