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Anti-ALDH7A1 Antibody (3P955)

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Catalog No. TMAY-01934
Alias PDE, EPD, ATQ1, aldehyde dehydrogenase 7 family, member A1

Anti-ALDH7A1 Antibody (3P955) is a Rabbit antibody targeting ALDH7A1. Anti-ALDH7A1 Antibody (3P955) can be used in WB,ELISA,IP.

Anti-ALDH7A1 Antibody (3P955)

Anti-ALDH7A1 Antibody (3P955)

😃Good
Catalog No. TMAY-01934Alias PDE, EPD, ATQ1, aldehyde dehydrogenase 7 family, member A1
Anti-ALDH7A1 Antibody (3P955) is a Rabbit antibody targeting ALDH7A1. Anti-ALDH7A1 Antibody (3P955) can be used in WB,ELISA,IP.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: WB, ELISA, IP

Reactivity: Human
AliasPDE, EPD, ATQ1, aldehyde dehydrogenase 7 family, member A1
Ig Type
Rabbit IgG
Clone
3P955
Reactivity
Human
Verified Activity
1. Anti-ALDH7A1 rabbit monoclonal antibody at 1:1000 dilution.
-Lane A: Hela Whole Cell Lysate.
-Lane B: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:58 kDa.
-Observed band size:58 kDa.
2. ALDH7A1 was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-2 µL anti-ALDH7A1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ALDH7A1 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution.
-Developed using the DAB staining technique.
-Performed under reducing conditions.
-Predicted band size: 55 kDa.
-Observed band size: 55 kDa
Application
WB,ELISA,IP
Recommended Dose
WB: 1:1000-1:5000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH7A1 (rh ALDH7A1; TMPY-01588; P49419-2; Ser 2-Gln 511).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human ALDH7A1 protein (TMPY-01588)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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