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Anti-ALDH7A1 Antibody (3P955) is a Rabbit antibody targeting ALDH7A1. Anti-ALDH7A1 Antibody (3P955) can be used in WB,ELISA,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $268 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Human |
Alias | PDE, EPD, ATQ1, aldehyde dehydrogenase 7 family, member A1 |
Ig Type | Rabbit IgG |
Clone | 3P955 |
Reactivity | Human |
Verified Activity | 1. Anti-ALDH7A1 rabbit monoclonal antibody at 1:1000 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:58 kDa. -Observed band size:58 kDa. 2. ALDH7A1 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -2 µL anti-ALDH7A1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ALDH7A1 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 55 kDa. -Observed band size: 55 kDa |
Application | WB,ELISA,IP |
Recommended Dose | WB: 1:1000-1:5000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ALDH7A1 (rh ALDH7A1; TMPY-01588; P49419-2; Ser 2-Gln 511). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | ALDH7A1 (Aldehyde dehydrogenase 7 family, member A1) is a member of subfamily 7 in the aldehyde dehydrogenase family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. Mammalian ALDH7A1 is homologous to plant ALDH7B1 which protects against various forms of stress such as increased salinity, dehydration and treatment with oxidants or pesticides. In mammals, ALDH7A1 is known to play a primary role during lysine catabolism through the NAD+-dependent oxidative conversion of aminoadipate semialdehyde (AASA) to its corresponding carboxylic acid, α-aminoadipic acid. Deleterious mutations in human ALDH7A1 are responsible for pyridoxine-dependent and folinic acid-responsive seizures. ALDH7A1 is a novel aldehyde dehydrogenase expressed in multiple subcellular compartments that protects against hyperosmotic stress by generating osmolytes and metabolizing toxic aldehydes. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human ALDH7A1 protein (TMPY-01588) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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