Antibody Type: Mouse Monoclonal

Application: ELISA, WB, FC

Reactivity: Human

IgG2b

6F523

Human

1. Western Blot
-Positive WB detected in: 293T whole cell lysate transfected with SEMA4D; Recombinant proteins with SEMA4D
-All lanes: SEMA4D antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 130, 150 KDa
-Observed band size: 130, 150 KDa
-Exposure time：5min
2. Overlay Peak curve showing 293T whole cell lysate transfected with SEMA4D stained with TMAH-01082 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FC

Monoclonal

Unconjugated

Recombinant Protein: Human SEMA4D Protein(22-734aa)

Human

10507

Signal Transduction