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EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc)

Catalog No. TMPK-00206

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 54.2 kDa and the accession number is P16422.

EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc)

EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc)

Catalog No. TMPK-00206
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 54.2 kDa and the accession number is P16422.
Pack SizePriceAvailabilityQuantity
100 μg$3027-10 days
500 μg$1,2107-10 days
1 mg$2,0107-10 days
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Product Information

Biological Activity
Immobilized Human EpCAM, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA.
Description
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 54.2 kDa and the accession number is P16422.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP16422
Synonyms
TROP1,TACSTD1,TACST-1,MOC31,MK1,MIC18,KSA,HNPCC8,gp40,ESA,EpCAM,EGP40,EGP314,EGP-2,EGP,DIAR5,CD326,ACSTD1
Construction
Gln24-Lys265
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight54.2 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.

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