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EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc)

(Synonyms: TROP1, TACSTD1, TACST-1, MOC31, MK1, MIC18, KSA, HNPCC8, gp40, ESA, EpCAM, EGP40, EGP314, EGP-2, EGP, DIAR5, CD326, ACSTD1) Copy Product Info

Synonyms: TROP1, TACSTD1, TACST-1, MOC31, MK1, MIC18, KSA, HNPCC8, gp40, ESA, EpCAM, EGP40, EGP314, EGP-2, EGP, DIAR5, CD326, ACSTD1

Catalog No. TMPK-00206 Copy Product Info
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 54.2 kDa and the accession number is P16422.

Batch Information

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Purity:> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human EpCAM, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA.
Description
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 54.2 kDa and the accession number is P16422.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberP16422
ConstructionGln24-Lys265
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsTROP1, TACSTD1, TACST-1, MOC31, MK1, MIC18, KSA, HNPCC8, gp40, ESA, EpCAM, EGP40, EGP314, EGP-2, EGP, DIAR5, CD326, ACSTD1
Research Background
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Chemical Properties
Molecular Weight54.2 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc) chemical structure | EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, hFc) molecular weight