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Anti-SEMA4D Antibody (2P72)

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Catalog No. TMAH-01081
Alias Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164

Anti-SEMA4D Antibody (2P72) is an antibody targeting SEMA4D. Anti-SEMA4D Antibody (2P72) can be used in ELISA, FC.

Anti-SEMA4D Antibody (2P72)

Anti-SEMA4D Antibody (2P72)

😃Good
Catalog No. TMAH-01081Alias Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164
Anti-SEMA4D Antibody (2P72) is an antibody targeting SEMA4D. Anti-SEMA4D Antibody (2P72) can be used in ELISA, FC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Monoclonal

Application: ELISA, FC

Reactivity: Human
AliasSemaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164
Ig Type
Human IgG4(S228P)
Clone
2P72
Reactivity
Human
Verified Activity
1. Untransfected HEK293T cells surface (green line) and transfected Human SEMA4D HEK293T stable cells surface (red line) were stained with anti-SEMA4D antibody (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
2. The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Sema4d at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 98.14-174.8 ng/mL.
3. The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 1.082-4.805 ng/mL.
Application
ELISA, FC
Recommended Dose
FC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Research BackgroundCell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human SEMA4D Protein
Antigen Species
Human
Gene ID
10507
Uniprot ID
Biology Area
Neuroscience, Immunology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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