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Anti-SEMA4D Antibody (2P72) is an antibody targeting SEMA4D. Anti-SEMA4D Antibody (2P72) can be used in ELISA, FC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, FC Reactivity: Human |
Alias | Semaphorin-4D, SEMAJ, SEMA4D, M-sema-G, MGC169141, MGC169138, FLJ46484, FLJ39737, FLJ34282, FLJ33485, coll-4, CD100, C9orf164 |
Ig Type | Human IgG4(S228P) |
Clone | 2P72 |
Reactivity | Human |
Verified Activity | 1. Untransfected HEK293T cells surface (green line) and transfected Human SEMA4D HEK293T stable cells surface (red line) were stained with anti-SEMA4D antibody (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry. 2. The Binding Activity of Mouse Sema4d with Anti-SEMA4D recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Sema4d at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 98.14-174.8 ng/mL. 3. The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 1.082-4.805 ng/mL. |
Application | ELISA, FC |
Recommended Dose | FC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human SEMA4D Protein |
Antigen Species | Human |
Gene ID | 10507 |
Uniprot ID | |
Biology Area | Neuroscience, Immunology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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