MERS-CoV Spike/S1 Protein (aa 1-725, His)

Name: MERS-CoV Spike/S1 Protein (aa 1-725, His)
Brand: TargetMol
SKU: TMPY-03574
Price: 68 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: RBD Prote, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus spike Protein, MERS-CoV, coronavirus s2 Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV) Copy Product Info

Synonyms: RBD Prote, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus spike Protein, MERS-CoV, coronavirus s2 Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV

Catalog No. TMPY-03574 Copy Product Info

MERS-CoV Spike/S1 Protein (aa 1-725, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 79.9 kDa and the accession number is K0BRG7.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	-	In Stock
10 µg	$108	-	In Stock
20 µg	$177	-	In Stock
50 µg	$347	-	In Stock
100 µg	$583	-	In Stock
200 µg	$993	7-10 days	7-10 days
500 µg	$2,010	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:93.3%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) at 2 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 . The EC50 of biotinylated Fc-DPP4 is 15-60 ng/ml.
Description	MERS-CoV Spike/S1 Protein (aa 1-725, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 79.9 kDa and the accession number is K0BRG7.
Species	MERS-CoV
Expression System	HEK293 Cells
Tag	C-His
Accession Number	K0BRG7
Construction	A DNA sequence encoding the MERS-CoV spike protein S1 Subunit (AFS88936.1)(Met1-Glu725) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Tyr 18
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	RBD Prote, ncov spike Protein, MERS-CoV, ncov s2 Protein, MERS-CoV, ncov s1 Protein, MERS-CoV, ncov RBD Protein, MERS-CoV, cov spike Protein, MERS-CoV, coronavirus spike Protein, MERS-CoV, coronavirus s2 Protein, MERS-CoV, coronavirus s1 Protein, MERS-CoV
Research Background	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Chemical Properties

Molecular Weight

79.9 kDa (predicted); 94 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.