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Anti-ALCAM Antibody (3O178)

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Catalog No. TMAH-00051
Alias MEMD, KG-CAM, CD6L, CD166, ALCAM

Anti-ALCAM Antibody (3O178) is an antibody targeting ALCAM. Anti-ALCAM Antibody (3O178) can be used in ELISA, WB, IHC.

Anti-ALCAM Antibody (3O178)

Anti-ALCAM Antibody (3O178)

😃Good
Catalog No. TMAH-00051Alias MEMD, KG-CAM, CD6L, CD166, ALCAM
Anti-ALCAM Antibody (3O178) is an antibody targeting ALCAM. Anti-ALCAM Antibody (3O178) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human, Mouse
AliasMEMD, KG-CAM, CD6L, CD166, ALCAM
Ig Type
Rabbit IgG
Clone
3O178
Reactivity
Human, Mouse
Verified Activity
1. Western Blot
-Positive WB detected in: PC3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate, Mouse brain tissue
-All lanes: ALCAM antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 66, 64, 16, 34 kDa
-Observed band size: 100 kDa
2. IHC image of TMAH-00051 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00051 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:2000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite.; [Isoform 3]: Secreted.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Required for normal hematopoietic stem cell engraftment in the bone marrow. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation. Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human ALCAM
Antigen Species
Human
Gene ID
214
Uniprot ID
Biology Area
Tags & Cell Markers, Immunology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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