Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 487.00 | |
500 μg | 5 days | $ 1,950.00 | |
1 mg | 5 days | $ 3,250.00 |
Description | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
Species | Human |
Expression System | HEK293 |
Tag | C-hFc |
Accession Number | P10696 |
Synonyms | GCAP, ALPPL, Alkaline phosphatase, placental-like, Alkaline Phosphatase, ALP-1, PLAP-like, ALPG, ALPPL2 |
Construction | Recombinant Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile20-Asp503.[Accession |P10696] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
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Please read the User Guide of Recombinant Proteins for more specific information.
Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) ALP 1 GCAP ALPPL Alkaline phosphatase, placental-like Alkaline Phosphatase ALP-1 ALPPL-2 ALP1 ALPPL 2 PLAP-like ALPG ALPPL2 recombinant recombinant-proteins proteins protein