Anti-Flag Tag Antibody (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody (3S989) can be used in ELISA, IP, WB.

IgG

3S989

DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins

1. Anti-Flag Tag Antibody (3S989) (TMAS-00123) binds with DYKDDDDK Tag.
Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
Anti-Flag Tag Antibody (3S989) (TMAS-00123) dilution start from 1,000 ng/ml.
EC50= 47.78 ng/ml.
2. Sensitivity and specificity of Anti-Flag Tag Antibody (3S989) (TMAS-00123) to DYKDDDDK-tag Fusion Protein by Western blot.
A: N-terminal DYKDDDDK-tagged fusion protein (25 ng, 10 ng, 5 ng)
B: C-terminal DYKDDDDK-tagged fusion protein (25 ng, 10 ng, 5 ng)
C: Internal positions DYKDDDDK-tagged fusion protein (50ng, 25 ng, 10 ng, 5 ng)
Primary Antibody: Anti-Flag Tag Antibody (3S989) (TMAS-00123) 0.2 μg/ml.
Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye800] 0.125 μg/ml.
3. Western blot analysis of immunoprecipitation from cell lysates containing DYKDDDDK fusion protein by using Anti-Flag Tag Antibody (3S989) (TMAS-00123).
Lane 1: Anti-Flag Tag Antibody (3S989) (TMAS-00123) (4 μg) + Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein.
Lane 2: Rabbit IgG (4 μg)+ Hela cell lysate containing N-terminal DYKDDDDK tagged fusion protein.
Lane 3: N-terminal DYKDDDDK tagged fusion protein (Input).
Lane 4: Anti-Flag Tag Antibody (3S989) (TMAS-00123) (4 μg) + Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein.
Lane 5: Rabbit IgG (4 μg)+ Hela cell lysate containing C-terminal DYKDDDDK tagged fusion protein.
Lane 6: C-terminal DYKDDDDK tagged fusion protein (Input).
Primary Antibody: Anti-Flag Tag Antibody (3S989) (TMAS-00123) 0.2 μg/ml.
Secondary Antibody:Goat anti-Rabbit IgG (H&L) [IRDye800] 0.125 μg/ml.

ELISA: 0.05-0.2 μg/ml; IP: 0.2-1 μg/ml; WB: 0.2-1 μg/ml

Monoclonal

Unconjugated

HRP/Biotin/FITC

4

KLH conjugated synthetic peptide: DYKDDDDK