Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, WB, IHC, IF

Reactivity: Human, Mouse

Mouse IgG2a

7S938

Human, Mouse

1. Western Blot
-Positive WB detected in: MCF7 whole cell lysate,JK whole cell lysate,K562 whole cell lysate,HepG2 whole cell lysate
-All lanes: HIST1H3A antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 16 kDa
-Observed band size: 16 kDa
2. IHC image of TMAH-00547 diluted at 1:300 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00547 diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
4. Immunofluorescence staining of HepG2 cell with TMAH-00547 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
5. Immunofluorescence staining of Hela cell with TMAH-00547 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

ELISA, WB, IHC, IF

WB:1:1000-1:5000; IHC:1:20-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human HIST1H3A Protein

Human

8350

Epigenetics and Nuclear Signaling