Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IF, FCM

Reactivity: Human

IgG1

6P67

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
-All lanes: TSG101 antibody at 1:500
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 44, 32 KDa
-Observed band size: 44 KDa
-Exposure time:5min
2. Immunofluorescence staining of U251 cells with TMAH-01216 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
3. Overlay Peak curve showing U251 cells stained with TMAH-01216 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IF, FCM

Monoclonal

Unconjugated

Recombinant Protein: Human TSG101 Protein (1-145AA)

Human

7251

Immunology