Claudin-6 Protein, Human, Recombinant (His & Avi), Biotinylated

Claudin-6 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 22.7 kDa and the accession number is P56747.

Pack Size	Availability	Price/USD	Quantity
20 μg	5 days	$ 3,020.00
100 μg	5 days	$ 10,610.00

Pack Size

Availability

Price/USD

Quantity

20 μg

5 days

$ 3,020.00

100 μg

5 days

$ 10,610.00

Description

Species	Human
Expression System	HEK293 Cells
Tag	His, Avi
Accession Number	P56747
Synonyms	claudin 6
Construction	The Human CLDN6 (P56747) (1-184) was expressed, with a polyhistidine tag and AVI tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Protein Purity	≥ 75 % as determined by SDS-PAGE.
Molecular Weight	22.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH 7.5 with glycerol as protectant.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -70℃ or lower. Samples are stable for up to 12 months at -80℃. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Claudin-6 (CLDN6) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and intracellular N and C tails. CLDN6 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It regulates the paracellular flux of cations in the kidney through forming heterotypic strands with other claudins and has a role in maintaining the lung epithelial barrier function. The CLDN6 gene is adjacent to another family member, CLDN9, on chromosome 16. Certain CLDN6-positive cancers, encompassing ovarian, endometrial, and testicular malignancies, have been shown to have their proliferation and invasiveness suppressed upon CLDN6 silencing.