Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF, FCM

Reactivity: Human

Rabbit IgG

8C308

Human

1. Western Blot
-Positive WB detected in: HT-29 whole cell lysate
-All lanes: TP53 antibody at 1:500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 43 kDa
-Observed band size: 43 kDa
2. Immunofluorescence staining of MCF-7 cell with TMAH-01204 at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Immunofluorescence staining of Hela cell with TMAH-01204 at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
4. Overlay Peak curve showing Hela cells surface stained with TMAH-01204 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min.Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IF, FCM

WB:1:1000-1:5000; IF:1:20-1:200; FCM:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human TP53 Protein

Human

7157

Epigenetics and Nuclear Signaling, Cancer, Cell biology