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Anti-ABCG2 Polyclonal Antibody
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Catalog No. TMAB-00024
Alias UAQTL1, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG 2, ABC15
Anti-ABCG2 Polyclonal Antibody is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-ABCG2 Polyclonal Antibody
Anti-ABCG2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00024Alias UAQTL1, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG 2, ABC15
Anti-ABCG2 Polyclonal Antibody is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ABCG2 Polyclonal Antibody is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Alias | UAQTL1, Placenta specific MDR protein, Placenta specific ATP binding cassette transporter, MXR1, MXR, Multi drug resistance efflux transport ATP binding cassette sub family G(WHITE) member 2, MRX, Mitoxantrone resistance associated protein, MGC102821, GOUT1, EST157481, CDw338 antigen, CDw338, CD338, Breast cancer resistance protein, BMDP, BCRP1, ATP-binding cassette sub-family G member 2, ATP binding cassette transporter G2, ATP binding cassette sub family G(WHITE) member 2, ABCP, ABCG 2, ABC15 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Mouse Placenta tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Mouse Lung tissue lysates Lane 4: Rat Placenta tissue lysates Lane 5: Rat Cerebrum tissue lysates Lane 6: Rat Lung tissue lysates Lane 7: Human MCF-7 cell lysates Lane 8: Human HepG2 cell lysates Lane 9: Human HUVEC cell lysates Primary: Anti-ABCG2 (TMAB-00024) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 82 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. Breast Cancer Resistance Protein (BCRP) is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone. |
| Immunogen | KLH conjugated synthetic peptide: human ABCG2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | MRP-related proteins,Drug metabolism,Drug metabolism,ATPases,Surface Molecules,Surface Molecules,Tumor Associated |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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