Anti-ABCG2 Polyclonal Antibody is a Rabbit antibody targeting ABCG2. Anti-ABCG2 Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-ABCG2/CD338 Polyclonal Antibody, Unconjugated (TMAB-00024) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human HepG2 cell lysates
Lane 9: Human HUVEC cell lysates
Primary: Anti-ABCG2 (TMAB-00024) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 82 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human ABCG2

Human

ABCG2

Broad substrate specificity ATP-binding cassette transporter ABCG2

MRP-related proteins,Drug metabolism,Drug metabolism,ATPases,Surface Molecules,Surface Molecules,Tumor Associated

Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.