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Anti-Phospho-RPS6KA1 (Thr359, Ser363) Antibody (2F344)
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Catalog No. TMAH-00960
Alias S6K-alpha-1, S6K alpha 1, RSK1p90, RSK1, RSK 1 p90, RSK 1, RSK, RPS6KA1 (p-Thr359, Ser363), RPS6KA1 (p-T359, S363), rps6ka, RPS6K1 alpha, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase 90kD polypeptide 1, ribosomal protein S6 kinase, p-RPS6KA1 (Thr359, Ser363), p-RPS6KA1 (T359, S363), pp90RSK1, polypeptide 1, Phospho-RPS6KA1 (T359, S363), p90S6K, p90RSK1, p90 RSK1, OTTHUMP00000004113, Mitogen-activated protein kinase-activated protein kinase 1A, MGC79981, MAPKAPK-1a, MAPKAPK1A, MAPKAP kinase 1a, MAP kinase activated protein kinase 1a, KS6A1_HUMAN, HU1, HU 1, EC 2.7.11.1, dJ590P13.1, 90kD, 90 kDa ribosomal protein S6 kinase 1
Anti-Phospho-RPS6KA1 (Thr359, Ser363) Antibody (2F344) is an antibody targeting Phospho-RPS6KA1 (Thr359, Ser363). Anti-Phospho-RPS6KA1 (Thr359, Ser363) Antibody (2F344) can be used in ELISA, WB, IF, IP.
Anti-Phospho-RPS6KA1 (Thr359, Ser363) Antibody (2F344)
Anti-Phospho-RPS6KA1 (Thr359, Ser363) Antibody (2F344)
😃Good
Catalog No. TMAH-00960Alias S6K-alpha-1, S6K alpha 1, RSK1p90, RSK1, RSK 1 p90, RSK 1, RSK, RPS6KA1 (p-Thr359, Ser363), RPS6KA1 (p-T359, S363), rps6ka, RPS6K1 alpha, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase 90kD polypeptide 1, ribosomal protein S6 kinase, p-RPS6KA1 (Thr359, Ser363), p-RPS6KA1 (T359, S363), pp90RSK1, polypeptide 1, Phospho-RPS6KA1 (T359, S363), p90S6K, p90RSK1, p90 RSK1, OTTHUMP00000004113, Mitogen-activated protein kinase-activated protein kinase 1A, MGC79981, MAPKAPK-1a, MAPKAPK1A, MAPKAP kinase 1a, MAP kinase activated protein kinase 1a, KS6A1_HUMAN, HU1, HU 1, EC 2.7.11.1, dJ590P13.1, 90kD, 90 kDa ribosomal protein S6 kinase 1
Anti-Phospho-RPS6KA1 (Thr359, Ser363) Antibody (2F344) is an antibody targeting Phospho-RPS6KA1 (Thr359, Ser363). Anti-Phospho-RPS6KA1 (Thr359, Ser363) Antibody (2F344) can be used in ELISA, WB, IF, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, IF, IP Reactivity: Human |
| Alias | S6K-alpha-1, S6K alpha 1, RSK1p90, RSK1, RSK 1 p90, RSK 1, RSK, RPS6KA1 (p-Thr359, Ser363), RPS6KA1 (p-T359, S363), rps6ka, RPS6K1 alpha, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase 90kD polypeptide 1, ribosomal protein S6 kinase, p-RPS6KA1 (Thr359, Ser363), p-RPS6KA1 (T359, S363), pp90RSK1, polypeptide 1, Phospho-RPS6KA1 (T359, S363), p90S6K, p90RSK1, p90 RSK1, OTTHUMP00000004113, Mitogen-activated protein kinase-activated protein kinase 1A, MGC79981, MAPKAPK-1a, MAPKAPK1A, MAPKAP kinase 1a, MAP kinase activated protein kinase 1a, KS6A1_HUMAN, HU1, HU 1, EC 2.7.11.1, dJ590P13.1, 90kD, 90 kDa ribosomal protein S6 kinase 1 |
| Ig Type | Rabbit IgG |
| Clone | 2F344 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in HepG2 whole cell lysate(treated with EGF or not) -All lanes Phospho-RPS6KA1 antibody at 0.69μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 90 KDa -Observed band size: 90 KDa 2. Immunofluorescence staining of Hela cells with TMAH-00960 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). 3. Immunoprecipitating Phospho-RPS6KA1 in Hela whole cell lysate -Lane 1: Rabbit control IgG(1μg)instead of TMAH-00960 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00960(3μg)+ Hela whole cell lysate(1mg) -Lane 3: Hela whole cell lysate (20μg) |
| Application | ELISA, WB, IF, IP |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB. Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-RPS6KA1 (T359+S363) |
| Antigen Species | Human |
| Gene ID | 6195 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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