Antibody Type: Mouse Monoclonal

Application: WB, ELISA, ELISA(Det), IHC-P, FCM, ICC/IF

Reactivity: Human, Rhesus, Cynomolgus

Mouse IgG1

7D257

Human, Rhesus, Cynomolgus;
Not React with: Mouse, Rat

1. Immunochemical staining of human TROP2 in human rectal cancer with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
2. Immunochemical staining of human TROP2 in human placenta with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of cytotrophoblast.
3. Immunochemical staining of human TROP2 in human mammary gland with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium.
4. Flow cytometric analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
5. Anti-TROP2 mouse monoclonal antibody at 1:500 dilution.
-Lane A: MCF7 Whole Cell Lysate.
-Lane B: NCI-N87 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:36 kDa.
-Observed band size:36-40 kDa.
6. Immunochemical staining of monkey TROP2 in monkey Palm skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
7. Immunochemical staining of human TROP2 in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
8. Immunochemical staining of human TROP2 in human hysterocarcinoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
9. Immunochemical staining of human TROP2 in human skin cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
10. Immunofluorescence staining of TROP-2 in NCI-N87 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with mouse anti-Human TROP-2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane.
11. Flow cytometric analysis of human TROP2 expression on NCI-N87 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
12. Immunochemical staining of human TROP2 in human thyroid cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF

WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000

Monoclonal

Unconjugated

Biotin/FITC/HRP/PE

5

Recombinant Protein: Human TROP2 protein (TMPY-01262)

Human

Cancer Drug Targets