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CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His)

(Synonyms: T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)) Copy Product Info

Synonyms: T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)

Catalog No. TMPY-03546 Copy Product Info
Immobilized CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His) at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3E Antibody (145-2C11) with the EC50 of 41.2 ng/ml determined by ELISA. (QC Test)

Product Information

Bioactivity
Bioactivity
Immobilized Recombinant Mouse CD3D & CD3E Heterodimer Protein (Cat#TMPY-03546) at 2 μg/mL (100 μL/well) can bind CD3D & CD3E Heterodimer Antibody, Rabbit MAb, the EC50 is 10-30 ng/mL.
Description
CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The accession number is P04235&P22646.
Species
Mouse
Expression System
HEK293 Cells
TagC-Flag, C-His
Accession NumberP04235 & P22646
ConstructionAsp22-Asp108 (CD3E) and Phe22-Ala105 (CD3D). Subunit CD3E is fused with a Flag tag at the C-terminus and subunit CD3D is fused with a His tag at the C-terminus.
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsT3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
Chemical Properties
Molecular Weight15.34 kDa (CD3E) and 14.81 kDa (CD3D) (Predicted); 18-22 kDa and 35-45 kDa (Due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His) chemical structure | CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His) molecular weight