Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.

CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 29.2 kDa and the accession number is P04235&P22646.

Mouse

HEK293 Cells

T3d,CD3d molecule, δ (CD3-TCR complex),CD3d molecule, delta (CD3-TCR complex)

A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Asp 22

> 85 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.