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Synonyms: T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $68 | 7-10 days | 7-10 days | |
| 10 µg | $108 | 7-10 days | 7-10 days | |
| 20 µg | $178 | 7-10 days | 7-10 days | |
| 50 µg | $357 | 7-10 days | 7-10 days | |
| 100 µg | $643 | 7-10 days | 7-10 days | |
| 200 µg | $1,150 | 7-10 days | 7-10 days | |
| 500 µg | $2,930 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Recombinant Mouse CD3D & CD3E Heterodimer Protein (Cat#TMPY-03546) at 2 μg/mL (100 μL/well) can bind CD3D & CD3E Heterodimer Antibody, Rabbit MAb, the EC50 is 10-30 ng/mL. |
| Description | CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The accession number is P04235&P22646. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-Flag, C-His |
| Accession Number | P04235 & P22646 |
| Construction | Asp22-Asp108 (CD3E) and Phe22-Ala105 (CD3D). Subunit CD3E is fused with a Flag tag at the C-terminus and subunit CD3D is fused with a His tag at the C-terminus. |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | T3d, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex) |
| Molecular Weight | 15.34 kDa (CD3E) and 14.81 kDa (CD3D) (Predicted); 18-22 kDa and 35-45 kDa (Due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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