Anti-CD9 Antibody (5F780) is a Mouse antibody targeting CD9. Anti-CD9 Antibody (5F780) can be used in ELISA, WB.

IgG1

5F780

Human

1. Western Blot
-Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MG-63 whole cell lysate, MCF-7 whole cell lysate
-All lanes CD9 antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 25 KDa
-Observed band size: 25 KDa
-Exposure time: 5min
2. Western Blot
-Positive WB detected in: A549 whole cell lysate at 20μg, 10μg, 5μg, 2.5μg whole cell lysate
-All lanes CD9 antibody at 1:2000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 25 KDa
-Observed band size: 25 KDa
-Exposure time: 5min
3. Western Blot
-Positive WB detected in: 20μg A549 whole cell lysate
-All lanes: CD9 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 25 KDa
-Observed band size: 25 KDa
-Exposure time: 5min
4. Western Blot
-Positive WB detected in: 1.Exosomes extracted from plasma
2.Exosomes extracted from serum
3.Exosomes extracted from Hela cells
-All lanes: CD9 antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 25 KDa
-Observed band size: 25 KDa
-Exposure time: 5min
5. IHC image of TMAH-00231 diluted at 1:50 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
6. IHC image of TMAH-00231 diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
7. Immunofluorescence staining of Hela cells with TMAH-00231 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
8. Immunofluorescence staining of HepG2 cells with TMAH-00231 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
9. Overlay histogram showing A549 cells stained with TMAH-00231 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
10. Overlay histogram showing Jurkat cells stained with TMAH-00231 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1(1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
11. Overlay histogram showing PC-3 cells stained with TMAH-00231 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
12. Overlay histogram showing U87 cells stained with TMAH-00231 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB

Monoclonal

Unconjugated

Recombinant Protein: Human CD9 antigen Protein (112-195AA)

Human

928

Cardiovascular