IL-4 Protein, Human, Recombinant (hFc)

Name: IL-4 Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPJ-00034
Rating: 4.5 (1 reviews)

(Synonyms: Pitrakinra, Lymphocyte Stimulatory Factor 1, Interleukin-4, IL-4, IL4, BSF-1, Binetrakin, B-Cell Stimulatory Factor 1) Copy Product Info

Synonyms: Pitrakinra, Lymphocyte Stimulatory Factor 1, Interleukin-4, IL-4, IL4, BSF-1, Binetrakin, B-Cell Stimulatory Factor 1

Catalog No. TMPJ-00034 Copy Product Info

IL-4 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 40-55 KDa and the accession number is P05112.

Pack Size	Price	USA Stock	Global Stock
5 µg	$80	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$198	7-10 days	7-10 days
50 µg	$390	7-10 days	7-10 days
100 µg	$626	7-10 days	7-10 days
200 µg	$987	7-10 days	7-10 days
500 µg	$1,900	7-10 days	7-10 days
1 mg	$2,730	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Loaded Cynomolgus IL-4RA-His on HIS1K Biosensor, can bind Human IL-4-Fc with an affinity constant of <10^-3 nM as determined in BLI assay. (Regularly tested). 2. Immobilized Human IL-4-Fc at 5μg/ml (100 μl/well) can bind Cynomolgus IL-4Ra-His . The ED50 of Recombinant Human IL-4Ra-His is 79.49 ng/ml. (Regularly tested);
Description	IL-4 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 40-55 KDa and the accession number is P05112.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P05112
Amino Acid	His25-Ser153
Construction	His25-Ser153
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Pitrakinra, Lymphocyte Stimulatory Factor 1, Interleukin-4, IL-4, IL4, BSF-1, Binetrakin, B-Cell Stimulatory Factor 1
Research Background	Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

Chemical Properties

Molecular Weight

40-55 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.