Anti-CD86 Polyclonal Antibody 3 is a Rabbit antibody targeting CD86. Anti-CD86 Polyclonal Antibody 3 can be used in FCM,ICC/IF,ELISA.

IgG

Human

1. The Raji (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CD86 antibody (TMAB-04088): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (CD86) polyclonal Antibody, unconjugated (TMAB-04088) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM: 1μg/Test; ICC/IF: 1:50-200; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: the human CD86

Human

CD86

T-lymphocyte activation antigen CD86

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.