Ephrin A5/EFNA5 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01748

Ephrin A5/EFNA5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is O08543-1.

Ephrin A5/EFNA5 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01748

Ephrin A5/EFNA5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is O08543-1.

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100 μg	$462	7-10 days

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Product Information

Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized Mouse EPHA5 at 2 μg/ml (100 μl/well) can bind mouse EFNA4 with a linear range of 1.28-32 ng/ml.
Description	Ephrin A5/EFNA5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.6 kDa and the accession number is O08543-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	O08543-1
Synonyms	RAGS,LERK-7,Epl7,ephrin-A5,EFL-5,AV158822,AL-1
Construction	A DNA sequence encoding the mouse EFNA5 isoform long (O08543-1) without the pro peptide (Met 1-Asn 203) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 21
Protein Purity	> 97 % as determined by SDS-PAGE
Molecular Weight	22.6 kDa (predicted); 28 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.