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Synonyms: IgE, Ig ε chain C region, Ig epsilon chain C region


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $123 | 7-10 days | 7-10 days | |
| 10 µg | $189 | 7-10 days | 7-10 days | |
| 20 µg | $293 | 7-10 days | 7-10 days | |
| 50 µg | $548 | 7-10 days | 7-10 days | |
| 100 µg | $873 | 7-10 days | 7-10 days | |
| 200 µg | $1,390 | 7-10 days | 7-10 days | |
| 500 µg | $2,580 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Human Fc epsilon RI alpha, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus IgE, His Avi Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). |
| Description | IgE Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is G8F4W7. |
| Species | Cynomolgus |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | G8F4W7 |
| Construction | Cys209-Lys429 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | IgE, Ig ε chain C region, Ig epsilon chain C region |
| Research Background | Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule. |
| Molecular Weight | 27.9 kDa (Predicted); 35-45 kDa (Reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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