5T4/TPBG Protein, Human, Recombinant (mFc)

Name: 5T4/TPBG Protein, Human, Recombinant (mFc)
Brand: TargetMol
SKU: TMPJ-00269
Rating: 4.5 (1 reviews)

(Synonyms: WAIF1 Protein, TPBG, M6P1 Protein, 5T4AG Protein, 5T4 Protein) Copy Product Info

Synonyms: WAIF1 Protein, TPBG, M6P1 Protein, 5T4AG Protein, 5T4 Protein

Catalog No. TMPJ-00269 Copy Product Info

5T4/TPBG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-110 KDa and the accession number is Q13641.

Pack Size	Price	USA Stock	Global Stock
5 µg	$81	7-10 days	7-10 days
10 µg	$130	7-10 days	7-10 days
20 µg	$198	7-10 days	7-10 days
50 µg	$393	7-10 days	7-10 days
100 µg	$638	7-10 days	7-10 days
200 µg	$987	7-10 days	7-10 days
500 µg	$1,980	7-10 days	7-10 days
1 mg	$2,970	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Loaded Human TPBG Antibody (5G9) (Human IgG1) on Protein-A Biosensor, can bind Human TPBG (C-mFc) with an affinity constant of 8.75 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human TPBG (C-mFc) at 2μg/ml (100 μl/well) can bind Human TPBG Antibody (5G9) (Human IgG1) . The ED50 of Human TPBG Antibody (5G9) (Human IgG1) is 1.57 ng/ml. (Regularly tested)
Description	5T4/TPBG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-110 KDa and the accession number is Q13641.
Species	Human
Expression System	HEK293 Cells
Tag	C-mFc
Accession Number	Q13641
Amino Acid	Ser32-Ser355
Construction	Ser32-Ser355
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	WAIF1 Protein, TPBG, M6P1 Protein, 5T4AG Protein, 5T4 Protein
Research Background	Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Chemical Properties

Molecular Weight

80-110 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.