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PD-L1 Protein, Human, Recombinant (His), Biotinylated

Catalog No. TMPY-04949
Synonyms: PDCD1LG1, B7H1, PD-L1, B7-H1, B7-H, CD274 molecule, PDL1, PDCD1L1

PD-L1 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is Q9NZQ7-1.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
PD-L1 Protein, Human, Recombinant (His), Biotinylated
Pack Size Availability Price/USD Quantity
20 μg In stock $ 197.00
100 μg 5 days $ 468.00
200 μg 5 days $ 797.00
500 μg 5 days $ 1,610.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Measured by its binding ability in a functional ELISA. Immobilized Human PD-1 His & hFc at 2 μg/ml (100 μl/well) can bind Human PD-L1 His, Biotinylated, the EC50 of Human PD-L1 His, Biotinylated is 50-300 ng/mL.

Description PD-L1 Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.8 kDa and the accession number is Q9NZQ7-1.
Species Human
Expression System HEK293 Cells
Tag C-His
Accession Number Q9NZQ7-1
Synonyms PDCD1LG1, B7H1, PD-L1, B7-H1, B7-H, CD274 molecule, PDL1, PDCD1L1
Construction The N-terminal segment (Met1-Thr239) of the extracellular domain of Human B7-H1 (NP_054862.1) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Protein Purity > 95% as determined by SDS-PAGE.

Molecular Weight 26.8 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Blockade: Blocking AntibodiImmune Checkpoint Blockade: PD-L1 / B7-H1 / CImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyPD-L1 / B7-H1 / CD274 Immune Checkpoint ProteTargeted Therapy

References and Literature

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Keywords

PD-L1 Protein, Human, Recombinant (His), Biotinylated PDCD1LG1 B7H1 PD-L1 B7-H1 PDL-1 B7-H CD274 molecule PDL1 PDCD1L1 PDL 1 recombinant recombinant-proteins proteins protein

 

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