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VISTA Protein, Human, Recombinant (hFc)
(Synonyms: VSIR, VISTA, SISP1, PP2135, PD-1H, PD1H, Gi24, Dies1, C10orf54, B7-H5, B7H5, 4632428N05Rik) Copy Product InfoSynonyms: VSIR, VISTA, SISP1, PP2135, PD-1H, PD1H, Gi24, Dies1, C10orf54, B7-H5, B7H5, 4632428N05Rik
Catalog No. TMPK-01185 Copy Product Info
B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.9 kDa and the accession number is Q9H7M9.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $42 | 7-10 days | 7-10 days | |
| 10 μg | $64 | 7-10 days | 7-10 days | |
| 20 μg | $98 | 7-10 days | 7-10 days | |
| 50 μg | $195 | 7-10 days | 7-10 days | |
| 100 μg | $323 | - | In Stock | |
| 200 μg | $587 | 7-10 days | 7-10 days | |
| 500 μg | $1,300 | - | In Stock |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Immobilized Human B7-H5, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
2. Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human IGSF11, His Tag with an affinity constant of 14.44 μM as determined in SPR assay. |
| Description | B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.9 kDa and the accession number is Q9H7M9. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-hFc |
| Accession Number | Q9H7M9 |
| Construction | Phe33-Ala194 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | VSIR, VISTA, SISP1, PP2135, PD-1H, PD1H, Gi24, Dies1, C10orf54, B7-H5, B7H5, 4632428N05Rik |
| Research Background |
| Molecular Weight | 44.9 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: VISTA Protein, Human, Recombinant (hFc) chemical structure | VISTA Protein, Human, Recombinant (hFc) molecular weight
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