VISTA Protein, Human, Recombinant (hFc)

Name: VISTA Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPK-01185
Rating: 4.5 (1 reviews)

(Synonyms: VSIR, VISTA, SISP1, PP2135, PD-1H, PD1H, Gi24, Dies1, C10orf54, B7-H5, B7H5, 4632428N05Rik) Copy Product Info

Synonyms: VSIR, VISTA, SISP1, PP2135, PD-1H, PD1H, Gi24, Dies1, C10orf54, B7-H5, B7H5, 4632428N05Rik

Catalog No. TMPK-01185 Copy Product Info

B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.9 kDa and the accession number is Q9H7M9.

Pack Size	Price	USA Stock	Global Stock
5 µg	$42	7-10 days	7-10 days
10 µg	$64	7-10 days	7-10 days
20 µg	$98	7-10 days	7-10 days
50 µg	$195	7-10 days	7-10 days
100 µg	$323	-	In Stock
200 µg	$587	7-10 days	7-10 days
500 µg	$1,300	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Immobilized Human B7-H5, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-B7-H5 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). 2. Human B7-H5, hFc Tag captured on CM5 Chip via Protein A can bind Human IGSF11, His Tag with an affinity constant of 14.44 μM as determined in SPR assay.
Description	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.9 kDa and the accession number is Q9H7M9.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q9H7M9
Construction	Phe33-Ala194
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	VSIR, VISTA, SISP1, PP2135, PD-1H, PD1H, Gi24, Dies1, C10orf54, B7-H5, B7H5, 4632428N05Rik
Research Background	B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces.

Chemical Properties

Molecular Weight

44.9 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.