Antibody Type: Recombinant Monoclonal

Application: ELISA, IF, FCM

Reactivity: Human

hIgG1

2Q771

Human

1. Immunofluorescence staining of hela cell with TMAH-00758 at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
2. Immunofluorescence staining of U251 cell with TMAH-00758 at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
3. Overlay Peak curve showing PC3 cells stained with TMAH-00758 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, IF, FCM

IF:1:50-1:200; FCM:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human MIF Protein

Human

4282

Immunology