CD8 alpha Protein, Human, Recombinant (His)

Name: CD8 alpha Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-06274
Rating: 4.5 (1 reviews)

(Synonyms: p32 186910, p32, MAL, Leu-2, Leu2, CD8a molecule, CD8 α, CD8 alpha, CD8) Copy Product Info

Synonyms: p32 186910, p32, MAL, Leu-2, Leu2, CD8a molecule, CD8 α, CD8 alpha, CD8

Catalog No. TMPY-06274 Copy Product Info

CD8 alpha Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 19 kDa and the accession number is P01732-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$129	7-10 days	7-10 days
10 µg	$213	7-10 days	7-10 days
20 µg	$345	7-10 days	7-10 days
50 µg	$695	7-10 days	7-10 days
100 µg	$1,380	7-10 days	7-10 days
200 µg	$1,590	7-10 days	7-10 days
500 µg	$2,690	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to bind biotinylated Beta-2 microglobulin Protein, Human, Recombinant (His Tag) in a functional ELISA.
Description	CD8 alpha Protein, Human, Recombinant (His) is expressed in CHO mammalian cells with His tag. The predicted molecular weight is 19 kDa and the accession number is P01732-1.
Species	Human
Expression System	CHO Cells
Tag	C-His
Accession Number	P01732-1
Construction	A DNA sequence encoding the extracellular domain of human CD8A (NP_001759.3) (Met1-Asp182) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Ser 22
Protein Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	p32 186910, p32, MAL, Leu-2, Leu2, CD8a molecule, CD8 α, CD8 alpha, CD8
Research Background	T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

Chemical Properties

Molecular Weight

19 kDa (predicted); 28 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.