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Anti-WFDC2 Antibody (7A490) is an antibody targeting WFDC2. Anti-WFDC2 Antibody (7A490) can be used in ELISA, WB, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, FCM Reactivity: Human |
Alias | WAP5, WAP four-disulfide core domain 2, HE4, EDDM4, dJ461P17.6 |
Ig Type | Rabbit IgG |
Clone | 7A490 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate -All lanes: HE4 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 25 kDa -Observed band size: 25 kDa 2. Overlay Peak curve showing MCF-7 cells stained with TMAH-01250 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, WB, FCM |
Recommended Dose | WB:1:500-1:2000; FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Secreted. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Broad range protease inhibitor. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human WFDC2 |
Antigen Species | Human |
Gene ID | 10406 |
Uniprot ID | |
Biology Area | Tags & Cell Markers |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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