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Anti-WFDC2 Antibody (7A490)

😃Good
Catalog No. TMAH-01250
Alias WAP5, WAP four-disulfide core domain 2, HE4, EDDM4, dJ461P17.6

Anti-WFDC2 Antibody (7A490) is an antibody targeting WFDC2. Anti-WFDC2 Antibody (7A490) can be used in ELISA, WB, FCM.

Anti-WFDC2 Antibody (7A490)

Anti-WFDC2 Antibody (7A490)

😃Good
Catalog No. TMAH-01250Alias WAP5, WAP four-disulfide core domain 2, HE4, EDDM4, dJ461P17.6
Anti-WFDC2 Antibody (7A490) is an antibody targeting WFDC2. Anti-WFDC2 Antibody (7A490) can be used in ELISA, WB, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, FCM

Reactivity: Human
AliasWAP5, WAP four-disulfide core domain 2, HE4, EDDM4, dJ461P17.6
Ig Type
Rabbit IgG
Clone
7A490
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate
-All lanes: HE4 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 25 kDa
-Observed band size: 25 kDa
2. Overlay Peak curve showing MCF-7 cells stained with TMAH-01250 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, WB, FCM
Recommended Dose
WB:1:500-1:2000; FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationSecreted.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundBroad range protease inhibitor.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human WFDC2
Antigen Species
Human
Gene ID
10406
Uniprot ID
Biology Area
Tags & Cell Markers
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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